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Real-time quantitative PCR Total RNA was extracted from aortic tissue and endothelial cells using TRIzol reagent (Takara Bio Inc., 9108), following the manufacturers instructions

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Injection Site Rotation Don't keep hitting the same spot

Was each component quantified separately

Copper is also used in superoxide dismutase, an enzyme that handles oxidative stress, and oxidative stress is one driver of fine lines and dull tone after years of UV exposure