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Importantly, there are numerous studies showing, through the regulation of the aforementioned transcription factors and more, that 4-HNE causes cell cycle arrest and inhibits the proliferation of malignant cells through the p53 and p21 dependent pathways (for review see ( Another important signalling metabolite that is generated through the cyclooxygenase-mediated peroxidation of PUFAs is malondialdehyde (MDA)

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Overall, increasing NAD + levels stimulated mitochondrial function, promoting mitophagy and preventing cellular senescence

The cells were subsequently stained with Dil membrane dye (S0033, Beyotime Biotechnology, China) for 15 minutes and Hoechst 33342 nuclear dye (14533, Sigma-Aldrich, USA) for another 15 minutes after fixation with 4% paraformaldehyde

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