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After TMB substrate solution is added, only those wells that contain Human MCP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color

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This release of histamine and tryptase increases blood flow to the skeletal muscles during exercise.[ref] Hypoxia has also been shown to induce mast cell degranulation.[ref] This is a normal response for most people, and the body can handle a certain increase in histamine and tryptase