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It showed that Val105 substitution results in steric restriction of the H-site due to shifts in the side chains of several amino acids leading to reduce the distance between G-site and H-site whereas in the Ile/Ile form such distance is increased suggesting less detoxification

Understanding the current evidence, acknowledging the limitations, and consulting with knowledgeable healthcare providers are all important steps

In addition to this transformative role, it also plays an important role in protecting cells from the negative effects of free radicals

Afterwards, SDS-PAGE gels were prepared and the protein samples were subjected to electrophoresis (80V for 30 min for concentrated gels and 120V for separated gels)