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(7) Oocytes were then rinsed 3 times in IBMX-free M2 (Sigma-Aldrich, Australia) containing 3 mg/ml BSA, which had been equilibrated for 24 h in 5% CO 2 , to clear off the IBMX from medium, then transferred into another clean, pH calibrated M16 medium that was overlayed with oil and returned to the incubator under 37 C in a humidified atmosphere of 5% CO 2 to assess subsequent meiotic development