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The mixture was then sonicated to form a suspension, centrifuged, and the supernatant was collected and concentrated to 10 mL

World J Gastroenterol 23:4658488 Farges M, Balcerzak D, Fisher B, Attaix D, Bchet D, Ferrara M, Baracos V (2002) Increased muscle proteolysis after local trauma mainly reflects macrophage-associated lysosomal proteolysis

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