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The N- and C-terminal regions of heavy metal-binding domains in typical P1B-type ATPases can associate to bind metal ions specifically like Cd 2 and Pb 2 162

doi: 10.1016/j.cgh.2020.05.021

The fluorescence signal can be measured with a fluorescence microplate reader at Ex/Em= 420/480 nm, which is directly proportional to the glutathione peroxidase activity

The epigenetic regulation through the methylation of DNA and histones may act blocking or activating transcription