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Germ-free mice showed increased bone mass, reduced CD4+ T cells and TNF- levels compared to normal counterparts, while FMT could restore these indexes to normal levels (117)

Dodle T, Mohanty D, Tripathy B, Panigrahy AB, Sirikonda S, Kumar L, et al

Abdalbari FH, Telleria CM

The cleavage of GST from the target protein can be challenging, especially if the cleavage site is not efficiently recognized by the protease or if the GST tag affects the accessibility of the cleavage site

Disulfide bonds are relatively stable under physiological conditions but undergo selective cleavage in the tumor microenvironment (TME), minimizing premature drug release [53]