HPLC measurement of DA, DOPAC and HVA DA and metabolites were separated by injecting 50 l of sample into a Microsorb C18 column (100 4.6 mm column, Analytical Column, Waters) and a mobile phase containing 130 mM NaH 2 PO 4 , 2 mM NaCl, 0.1 mM EDTA, 2 mM OSA (1-octanesulphonic acid sodium) and 12.5% (v/v) methanol, pH 3.71, at a flow rate of 1 ml min 1
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Peptide drugs on average have oral bioavailability below 1-2%
Discovery of a cyclic cell-penetrating peptide with improved endosomal escape and cytosolic delivery efficiency
doi:10.1001/jamainternmed.2016.6374.