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By preventing G-actin from polymerizing into F-actin, TB-500 maintains a pool of unpolymerized actin that allows cells, such as keratinocytes and fibroblasts, to physically "crawl" to the site of an injury

34 A derivatization procedure was used using phthalaldehyde

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Cytokines and chemokines including TNF-, IL-6, and IL-1 cross BBB and induce neuroinflammation directly, and also by activating inflammatory, glial, and neuronal cells in the brain (Banks et al., 2016)

After washing with double distilled water, the sections were dehydrated with 70% alcohol, 80% alcohol, and 95% alcohol for 2 min