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Primary hippocampal neuron cultures are used to study Dihexa's proposed effects on dendritic spine density and synaptic protein expression, typically measuring morphological changes via fluorescence microscopy and quantifying synaptic marker expression (such as PSD-95 or synaptophysin) relative to untreated controls

Each time you insert a needle to draw a dose, you introduce potential contamination from the needle surface or air without a preservative, bacterial colonies multiply with each puncture