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Immunostaining was performed by seeding cells on glass coverslips, fixing with paraformaldehyde, permeabilizing with Triton X-100, and then incubating with primary anti-NF-B p65 antibody, followed by Alexa Fluor 546-conjugated secondary antibody

After three days of streptozotocin injection, diabetic rats were checked for blood glucose levels from tail tip samples under non-fasted conditions using blood glucosemeter (Accu-check Roche Diagnostics co., USA)