The authors show that LXA4 activates the expression of GPX4, lysophosphatidic acid receptor-3 (LPAR3), and estrogen receptor beta (ESR2) in FLSs, which inhibits ferroptosis and reduces inflammation and chondrocyte matrix breakdown
10.1126/science.1172702 50
Subsequently, samples were concentrated to dryness in vacuum before dissolving in 150 L of 80% methanol (precooled at -20C), then centrifuged again at 13 500 g and 4C for 10 min to obtain the supernatant for high-performance liquid chromatography Ultimate 3000 (Thermo Fisher Scientific, USA)-tandem mass spectrometry Q Exactive Focus (Thermo Fisher Scientific, USA) (LC-MS/MS) analysis (95, 96)
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