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Although recent evidence indicates that these perilipins do not have to be phosphorylated in order to mediate translocation of HSL from the cytosol to the surface of the lipid droplet, phosphorylation does appear to be necessary for attainment of maximally stimulated lipolysis following PKA activation (84)

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After washing, the samples were incubated with secondary antibody

doi:10.1111/dom.14883