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K-means clustering Five clusters were used for k-means clustering, per the results of the sum of squared estimate of errors curve, on the ASV relative abundance data with clustering by animal (n = 25)

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Another important form of NO-related posttranslational modification (PTM) is protein S-nitrosylation (SNO), a product of the interaction of NO with the sulfhydryl groups of cysteine which leads to the formation of S-nitrosothiols [147, 159]