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Gund R, Christiano AM (2023) Impaired autophagy promotes hair loss in the C3H/HeJ mouse model of alopecia areata

These findings indicate that SMCs, like innate immune cells, can undergo trained immunity and maintain a long-term activated state

Purification performance of Glutathione 4FF resins. 50 mL of E.coli lysate containing a GST-tagged fusion protein was loaded on 0.5 mL packed Glutathione 4FF resins at 0.5 mL/mL. The column was then washed with 10 mL of PBS. The target GST-tagged protein was eluted with 7.5 mL of elution buffer (50 mM Tris-HCl, 10 mM GSH, pH 8.0). Fractions of lysate, flow-through, and eluate were analysed by SDS-PAGE. Lanes: (1) Raw cell lysate; (2) Flow-through; (3) Eluate

This results in the recruitment and activation of Parkin at the OMM, where it ubiquitinates multiple mitochondrial surface proteins