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both antibiotic use and germ-free conditions suppress the expression of CYP7A1 and CYP27A1, key enzymes involved in BA production based on animal mechanistic studies ( Clostridium and Bifidobacterium , as observed in human associative studies (Mulak, 2021)

They found negative correlations between clinical symptom scores and metabolites involved in redox metabolism, as well as neurotransmitters in patients treated with sulforaphane