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Immunohistochemistry For immunohistochemistry, paraffin sections of liver and intestine specimens were deparaffinized, rehydrated in decreasing concentrations of ethanol, treated for 10 minutes with 3% H 2 O 2 to quench endogenous peroxidase activity and heated to 100C for 1 h in 10 mM sodium citrate buffer, pH 6, for antigen retrieval

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Advantages: Fast absorption Reliable delivery Suitable for deficiency treatment Possible discomfort: Mild soreness afterwards Slight muscle ache Subcutaneous (SC) Injection This method injects Vitamin B12 into the fatty tissue beneath the skin

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