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Urinary metabonomics study of anti-depressive mechanisms of Millettia speciosa Champ on rats with chronic unpredictable mild stress-induced depression

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siRNA knockdown Macrophages (2.5 10 5 cells/mL) were seeded for 24 h and then were transfected with 10 nM of small interfering RNA (siRNA, GPx1 s804, s805, Thermo Fisher Scientific) or a negative control (Silencer Select siRNA Negative control 4394803, Thermo Fisher Scientific) following the manufacturers instructions

Much of Noopept cognitive effect may be mediated through its metabolite cycloprolylglycine (cPG), which concentrates preferentially in the hippocampus and has been shown to reduce hippocampal cell death, increase BDNF mRNA, and modulate inflammatory cytokine expression

Diagnostic information was reviewed and verified by an experienced child neurologist (SHM)