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Meanwhile, cells were also fixed with 4% paraformaldehyde solution and stained with crystal violet (CV) for 1 min, and optical images were obtained using the above optical microscope

Centrifuge samples at 1,000g for 15 minutes at 28C within 30 minutes of collection

Store the liquid solution strictly between 2C and 8C

Additionally, GPX3 can interact with GSH and thioredoxin reductase, or with thioredoxin alone, to produce electrons for GSH in the presence of GSH depletion (18), which in turn protects the cells from ROS-induced deoxyribonucleic acid (DNA) and cellular damage (15)

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