Benzyl alcohol at 0.9% concentration is bacteriostatic rather than bactericidal, which is sufficient to maintain sterility in a sealed vial environment during the research window.
What the "recipe" involves The formulation described across pharmaceutical references and community forums is straightforward: Sterile water for injection (not distilled water, not purified water sterile water for injection, USP) Benzyl alcohol at 0.9% concentration by volume (9 mg/mL) Combined in a sterile, pyrogen-free container under aseptic conditions The process people describe online typically involves: Sourcing sterile water for injection (sometimes substituted with distilled water a critical error) Calculating the benzyl alcohol volume (0.9 mL per 100 mL of final solution, or 0.27 mL per 30 mL) Combining in a vial or container using a syringe and filter needle Sealing and storing refrigerated On paper, this looks manageable
All injections were performed under general anesthesia using ultrasound guidance by a trained physiatrist under sterile conditions
I keep my vials, syringes, and a bag of alcohol pads neatly organized, making the process quick and hygienic
Breastfeeding Vitamin B12 is known to be distributed in human milk
