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Crystallization of Pt GSTF8 in presence of ()-catechin and GSH Purified Pt GSTF8 in 30 mM Tris, pH 8, 200 mM NaCl, 1 mM EDTA was concentrated to 12.4 mg ml 1 using an Amicon Ultra-4 device (10 kDa cut-off) (Merck Millipore) and supplemented with 1 mM GSH and 25 mM ()-catechin immediately before crystallization

GST is able specifically to bind to GSH or GSH analogs via the G-site, which catalyzes the interaction between GST amino-acid residues with GSH sulfhydryl and conventional electrophilic substances at the H-sites to promote catalytic action

[39] [40] RSSR' + 2 GSH RSH + R'SH + GSSG where R and R' are parts of the micro-nanogel structure, and GSSG is oxidized glutathione (glutathione disulfide)

Cell 2014;156:31731

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