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The fresh pig costal cartilage was cut into a rectangular bar (15 mm 40 mm) before washing with 1 M sodium hydroxide 3 times and rinsed in deionized water to remove adipose tissue

These mechanisms contribute to its broad regenerative effects observed in animal models across musculoskeletal, gastrointestinal, and systemic systems

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GHK-Cu is a naturally occurring copper-binding peptide first identified in human plasma