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Restriction-minus [mcrA
coli replicate at single-copy number from the F-factor replicon because expression of the trfA gene is repressed
BAC-Optimized Replicator v2
Ligate single-stranded adenylated DNA or RNA oligos to RNA targets
subcloning
Endura-Chemically Competent Cells (DUOs) size:24 rxns Restriction-minus [mcrAEndura Competent Cells Clone repetitive sequences and lentiviral libraries Stabilize direct repeats and create lentiviral constructs Generate lentiviral guide RNA libraries Recommended in CRISPR GeCKO library protocols Choose electrocompetent or chemically competent cells Highest efficiency commercially available cells for lentiviral cloning: over 1 107 cfu g (chem) or 1 1010 cfu g (electro) Transformation of Endura Electrocompetent Cells with GeCKO