SsCm1 SP -HA, SsCm2-HA, GmCms-HA, and UmCmu1 SP -HA (positive control) were cloned into pGEX-6P-1 vector, respectively, and the proteins were obtained in Escherichia coli BL21 and purified by glutathione affinity purification (C600327, Sangon)
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After stirring 1 h, the solvent was removed under reduced pressure, the organic layer was extracted with ethyl acetate (15 ml), and then washed with 2 5 ml of 10% citric acid, 2 5 ml NaHCO3 (1M), and then with 2 5 ml brine
Whether declining MOTS-c is a cause or consequence of this deterioration is still being resolved, but the correlation is striking enough that some researchers have proposed plasma MOTS-c as a biomarker of mitochondrial vitality and metabolic age