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Secondly, we have shown that a neurotoxic product of TRP metabolism, 3-OHKY, predicts severity of clinical symptoms during the early phase of illness and before exposure to antipsychotic drugs ( Lastly, optimum levels of purine metabolites have been associated with the dynamics of clinical symptoms and therapeutic improvements, which may lead to discovery of novel targets for drug development

Western blotting Cell pellets were lysed in RIPA buffer (25 mM Tris-HCl, pH = 7.5, 150 mM NaCl, 0.1% Nonidet P-40, 0.5% sodium deoxycholate and 0.1% SDS) supplemented with protease inhibitor cocktail (Sigma) and phosphatase Inhibitor Cocktail 2 and 3 (Sigma)

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Psychoneuroendocrinology 51:164175

Individual responses vary depending on baseline nutrient status and goals