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Figure 1 Agrobacterium-Mediated Transient Expression in Nicotiana benthamiana The plant expression vectors pEAQ- HT -DEST1/RFP and pEAQ- HT -DEST1/Lac51 were introduced into ElectroMAX Agrobacterium tumefaciens LBA4404 cells (Invitrogen, United States) by electroporation using a BTX ECM 630 electroporator (BTX-Division of Harvard Apparatus, United States), with the parameters 2.0 kV, 200 , and 25 F, as described by Clarke et al