Compared with GPX1-3, GPX4 is also involved in the reduction of complex membrane-associated phospholipid hydroperoxides such as phosphatidylcholine hydroperoxide and cholesterol hydroperoxide, in addition to reacting with soluble, low molecular weight hydroperoxides such as t-butyl hydroperoxide and cumene hydroperoxide [68]
It was derived from a protective sequence found naturally within human gastric juice protein
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HETEROLOGOUS EXPRESSION OF AsGGT1, AsGGT2, AND AsGGT3 IN YEAST The coding regions of AsGGT1 , AsGGT2 , and AsGGT3 were amplified by PCR using the cloned cDNA fragments described above, KOD plus DNA polymerase (Toyobo), and the following gene-specific primers: AsGGT1-FKpn3A (5- GGTACC AAAATGAACCAAATGGCGCCGGCTTC-3) and AsGGT1-stop-RXh (5- CTCGAG CTATACACAAGCAGGACTTC CATC-3) for AsGGT1
