Participants were also excluded based on psychoactive drug or investigational medication use, supplements that might affect brain GSH (e.g., N-acetylcysteine), pregnancy, special diet regimens (e.g., vegan), and MR contraindications including devices, foreign objects, or claustrophobia
Equal amounts of protein were resolved on SDS-polyacrylamide gel, transferred to nitrocellulose membrane (Amersham Biosciences, Milan, Italy), and then treated with the following primary antibodies: anti-Atg5 rabbit monoclonal (Cell Signaling Technology, MA, USA), anti-Atg7 rabbit monoclonal (Cell Signaling Technology, MA, USA), anti-LC3A/B rabbit monoclonal (Cell Signaling Technology, MA, USA), anti-SQSTM1/p62 rabbit monoclonal (Cell Signaling Technology, MA, USA), anti-Beclin-1 rabbit monoclonal (Cell Signaling Technology, MA, USA), anti-Phospho-S6 Ribosomal Protein rabbit monoclonal (Cell Signaling Technology, MA, USA), anti-S6 Ribosomal Protein mouse monoclonal (Cell Signaling Technology, MA, USA), anti-Phospho-AMPK rabbit monoclonal (Cell Signaling Technology, MA, USA), anti-AMPK mouse polyclonal (Cell Signaling Technology, MA, USA), anti-Cyclin D1 (Dako, Milan, Italy)
Asefa Z, et al
They also have some valuable and helpful clinical markers like calprotectin which can help rule in or out irritable bowel disease, and zonulin, a marker of leaky gut
M., Austin, D
