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Dispose of unused material in accordance with institutional laboratory safety protocols

Cell pellets were suspended in 400 l PBS, supplemented with 20 l of MK4-d 7 (2 ng l 1 in ethanol, 25709, Cayman) as internal standard, and sonicated for 30 s with a sonication probe (Bronson Sonifer)

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Since cortisol is a wakefulness hormone, a spike in the evening could certainly disrupt sleep patterns