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The hCTR cells were cultured in growth medium consisting of Dulbeccos Modified Eagles Medium (DMEM, Gibco, #31966-021) supplemented with 10% v/v foetal bovine serum (FBS, Gibco, #16140-071), 1% v/v Penicillin-Streptomycin (Gibco, #15140-122), 0.6 mg/mL Geneticin G418 (Gibco, #10131-027) and 0.2 mg/mL Hygromycin (Invitrogen, #10687010)
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This preservative helps reduce bacterial growth after first puncture, making BAC Water more suitable for repeated withdrawals from the same vial
Studies on osteoblastic cell behavior showed that Tb4 promotes both adhesion and proliferation of osteoblasts through focal adhesion signaling and ERK1/2 activation
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