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10.1038/s41531-019-0100-x 477 WengH.DengL.WangT.XuH.WuJ.ZhouQ.et al

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The eggs were then pelleted, washed three times with M9 buffer, and counted to ensure consistency across experiments

For GSH assay, cells were treated in Earles balanced salt solution (EBSS), supplemented with 25 mM glucose, 4 mM glutamine, 1 X MEM vitamin solution (Life technologies, CA, USA), 0.22% wt/vol NaHCO 3 , 1 mM sodium pyruvate, 0.2% BSA, 25 mM HEPES, 100 U/ml penicillin and 100 g/ml streptomycin in the presence or absence of 1 X MEM amino acid solution (Gibco, CA, USA) with or without 100 M cystine

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