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Following three 10 min washes in PBS-T, the membrane was incubated with HRP conjugated secondary antibodies, goat anti-rabbit (Thermo Scientific, 656120, 1:5000 dilution), or goat anti-mouse IgG antibody (Thermo Scientific, 626520, 1:5000 dilution) in blocking buffer for 1 hour at room temperature

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[145] [146] Chronic nicotine use also leads to accumulation of the transcription factor FosB in dopamine D1-type medium spiny neurons of the nucleus accumbens, a process implicated in sustained reward pathway modifications

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