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Animal models are useful for measuring physiological responses (e.g., reflexes, blood flow, tissue oxygen content, metabolic derangements)

Nitric oxide increases oxidative phosphorylation efficiency

Western blotting Total protein was extracted from paraformaldehyde-fixed, paraffin-embedded pancreatic tissues using the FFPE Total Protein Extraction Kit (Sangon Biotech, Shanghai, China) or from cultured PSCs using RIPA Lysis Buffer (Beyotime)

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