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Other toxic substances, like certain volatile organic compounds (VOCs) and even some heavy metals, can also be present in e-cigarette vapours (from various sources, including the e-liquid itself, the e-cigarette device, and even through secondary reactions), and in industrial environments
It slowly filled in with mud, and fossils of plants, fish, birds and crocodiles have been found there
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RNA was isolated using RNeasy kit (Qiagen) following the manufacturers protocol