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Together, we look closely at whether the trial is a good fit for our patients and the locations where it may be offered

Cells were seeded into a 384-well glass-bottom black plate (IBL, P384-1.5H-N) and left to adhere overnight

Patients should maintain adequate fluid intake based on individual needs, with the goal of maintaining pale yellow urine color

Raw reads were trimmed by quality score (min Phred score 35 from both ends and trimmed reads shorter than 25 bp were discarded)

Those with a previously affected pregnancy were also advised to receive 4,000 g (4 mg) of folic acid daily in order to reduce NTD recurrence (38)