Tong et al., 2016)
The colon has the largest microbial load in the body and contains about 10 14 bacteria with the dominant phyles of Firmicutes (renamed Bacillota), Bacteroidetes (Bacteroidota), Actinobacteria (Actinomycetota), and Proteobacteria (Pseudomonadota) ( Enterococcus , Lactobacillus , Ruminicoccus , Bacillus , and Clostridium , representing 95% of the Firmicutes phylum
If the solution, which should be perfectly clear, becomes cloudy, changes color, or has visible particulates, it should be discarded immediately as it may be contaminated or degraded
Table 2: The experimentally elucidated values of k, the (observed) fluorescence degradation rate constants and K, the rate constant related to hydrogen peroxide concentration
change can not be quick