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Then, 3 l of NH 4 HCO 3 (1 M) and 1 U alkaline phosphatase (Sigma) were added, followed by an additional two hours of incubation at 37 C

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The CPMG spectra were processed and multivariate statistical analyses (unsupervised principal component analysis, PCA and the supervised orthogonal partial least squares discriminant analyses, OPLS-DA), were performed using Metaboanalyst 6.0 software

D-Glucose prevents glutathione oxidation and mitochondrial damage after glutamate receptor stimulation in rat cortical primary neurons

Briefly, the initial small increase of SHs stimulates GH secretion in the early phase of puberty, whereas during late puberty, the further increase of SHs determines bone ossification and, therefore, the arrest of growth [18]