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2) Resuspend the cells in 1/10 volume of Lysis Buffer with appropriate amount of protease inhibitors added, which must have no effect on the binding capacity of agarose

Zwerger and Hirt, 2001

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Although our primary hypothesis concerns glutathione and glutamate, the underlying hypothesis is that in the residual state there is diminished neural metabolic integrity arising from a toxic process in the acute phase of illness [4]