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Factors underlying prefrontal and insula structural alterations in smokers

To explore sperm-associated epigenetic characteristics following nicotine exposure and cessation, sperm DNA methylation status was analyzed using high-performance liquid chromatography (HPLC) for quantifying 5-methylcytosine (5mC)

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RT-qPCR-based assays target the nucleic acid 18,19 and require sophisticated laboratory equipment and facilities