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Only an upper endoscopy procedure can allow the doctor to take a sample of the tissue from the esophagus to confirm this diagnosis, as well as to look for changes of potential dysplasia that cannot be determined on endoscopic appearance alone

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Cells were subsequently washed three times with PBS, fixed with 4% (w/v) formaldehyde for 30 min, washed again, and incubated with 50 M Comaz for 1 h at 37 C with shaking