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In contrast, LUHMES cells express a substantial amount of synuclein during differentiation into post-mitotic DA neurons by use of tetracycline (to switch-off the tetracycline-responsible myc gene), cyclic AMP, and GDNF (Lotharius et al., 2002, 2005)

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There is much variability in the outcomes of documented studies: some have recorded antibody development in response to the administration of recombinant human GDNF, a randomized trial detailed reactions to the placebo, and one study noted that subjects experienced strong adverse effects to intraventricular delivery of GDNF [33,34,62,65]

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