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Add 10 mL of 13% perchloric acid solution, mix well, and let stand for 20 minutes

In addition to directly affecting mTOR, activation of AMPK may also affect the activity of STAT3 (signal transduction and transcriptional activator 3) (84, 87)

In some embodiments, the otic formulation or composition comprises between about 0.10% to about 2% by weight of the therapeutic agent, or pharmaceutically acceptable prodrug or salt thereof