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For this example, we'll stick with 2 mL

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These enzymes function by conjugating the small molecule glutathione to toxic compounds, making them more soluble and easier for the body to excrete

Importantly, affinity chromatography utilizes a specialized filtered, column-like matrix, and methods are best suited for larger amounts of starting samples or when larger amounts of purified protein are necessary

doi: 10.1007/s00228-020-02919-8