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In HEK293T cells, UBE2O overexpression leads to a dose-dependent reduction of endogenous BMAL1, while its knockdown increases BMAL1 protein levels ( UBE2O overexpression attenuates BMAL1-driven transcription and dampens downstream circadian outputs, while its silencing increases PER2 rhythm amplitude, confirming its role in maintaining clock dynamics (Maffeo and Cilloni, 2024)

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These findings demonstrate that the methylation levels at CpG sites in the CLOCK and PER2 genes were positively correlated with the degree of weight loss in obese women, indicating that some epigenetic changes are associated with the phenotypes of obesity and diabetes

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