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3 mM CuCl 2 solutions prepared in the same buffer were titrated into the peptide solution at molar ratios of 0, 1, 2, 3, 4, and 5 using the titration syringe

Detoxification The liver and kidneys use glutathione to detoxify harmful substances

The side-chain hydroxyl oxygen of the glutamate part chelates with CuA at a distance of 2.66 , while the carbonyl oxygen forms a hydrogen bond with S361