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The PVDF membranes were incubated with primary antibodies overnight at 4 C, incubated with secondary antibodies at room temperature for 1 h, and visualized using the Bio-Rad ChemiDoc XRS + system

Peptides for Pets, Hype, Hope, and What You Must Know Peptides are trending everywhere, from human anti-aging clinics to elite dog rehab centers, and like anything trendy, they come with big promises and even bigger confusion

Do not use sterile water (which lacks preservative and must be used within 24 hours) or normal saline

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Front Immunol (2021) 12:611711