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Human RCC2 was amplified from U-2 OS cDNA and subcloned into a peGFP-C1 vector (Clontech) using restriction digest (SmaI, EcoRI) and Gibson assembly

however, this is FDA staff's proposed position going into the meeting, not a final action, and the committee's discussion, any vote, and FDA's ultimate decision are still pending, so the outcome of the review is not yet known

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Women with higher HEI scores had higher vitamin C intakes than those with lower scores with a trend for a protective association of vitamin C intake from foods alone, but not from a combination of foods and supplements, suggesting that vitamin Ccontaining foods rather than vitamin C itself may afford protection from nuclear cataract [130]

Spillover may engage ligand-gated mechanisms also at nearby dendrites, including dendrites of other cells within the spillover area, but still within a local region around the activated synapses